Levene , Analysis at High Concentrations Zero - Mode Waveguides for Single - Molecule
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http://www.sciencemag.org/cgi/content/full/299/5607/682 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/299/5607/682/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.sciencemag.org/cgi/content/full/299/5607/682#related-content http://www.sciencemag.org/cgi/content/full/299/5607/682#otherarticles , 7 of which can be accessed for free: cites 20 articles This article 124 article(s) on the ISI Web of Science. cited by This article has been http://www.sciencemag.org/cgi/content/full/299/5607/682#otherarticles 21 articles hosted by HighWire Press; see: cited by This article has been http://www.sciencemag.org/cgi/collection/chemistry Chemistry : subject collections This article appears in the following
منابع مشابه
Zero-mode waveguides for single-molecule analysis at high concentrations.
Optical approaches for observing the dynamics of single molecules have required pico- to nanomolar concentrations of fluorophore in order to isolate individual molecules. However, many biologically relevant processes occur at micromolar ligand concentrations, necessitating a reduction in the conventional observation volume by three orders of magnitude. We show that arrays of zero-mode waveguide...
متن کاملConcentrations Zero - Mode Waveguides for Single - Molecule Analysis at High
, 682 (2003); 299 Science et al. M. J. Levene Concentrations Zero-Mode Waveguides for Single-Molecule Analysis at High This copy is for your personal, non-commercial use only. clicking here. colleagues, clients, or customers by , you can order high-quality copies for your If you wish to distribute this article to others here. following the guidelines can be obtained by Permission to republish ...
متن کاملSingle-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).
Resolving single fluorescent molecules in the presence of high fluorophore concentrations remains a challenge in single-molecule biophysics that limits our understanding of weak molecular interactions. Total internal reflection fluorescence (TIRF) imaging, the workhorse of single-molecule fluorescence microscopy, enables experiments at concentrations up to about 100 nM, but many biological inte...
متن کاملAnalysis at High Concentrations Zero - Mode Waveguides for Single - Molecule
http://www.sciencemag.org/cgi/content/full/299/5607/682 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/299/5607/682/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://w...
متن کاملSingle molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides.
Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to approximately 17 mm/s. We derive an analytic scaling relationship [equat...
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